Composite

Part:BBa_K2557024

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


Ubc promoter--PhiC31-RDF

According to the feedback of digital model, the recombination efficiency and expression level of recombinase-RDF will affect the stability of the switch we designed. Therefore, we need to characterize the promoters of different intensities and RDF, and hope to get an optimal combination scheme. The Ubc promoter and PhiC31-RDF are characterized here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 408
    Illegal BamHI site found at 1319
    Illegal BamHI site found at 1405
    Illegal BamHI site found at 1652
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 486
    Illegal NgoMIV site found at 715
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 2328
    Illegal AgeI site found at 414
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1011
    Illegal BsaI.rc site found at 1414
    Illegal BsaI.rc site found at 1767
    Illegal BsaI.rc site found at 2409
    Illegal SapI.rc site found at 3011


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